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STEMCELL Technologies Inc rosetteseptm human t cell enrichment cocktail
Rosetteseptm Human T Cell Enrichment Cocktail, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rosetteseptm+human+t+cell+enrichment+cocktail/rosettesep+human+b+cell+enrichment+cocktail/pmc12272151-50-20-26
Average 90 stars, based on 1 article reviews
rosetteseptm human t cell enrichment cocktail - by Bioz Stars, 2026-09
90/100 stars

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Isolation:

Article Title: Mesothelin-based CAR-T cells exhibit potent antitumor activity against ovarian cancer
Article Snippet: .. Human peripheral blood mononuclear cells (PBMC) were collected from healthy donors with informed consent and isolated using RosetteSepTM Human T Cell Enrichment Cocktail (STEMCELL, Canada) according to the manufacturer’s protocol. .. T cells were cultured in advanced 1640 media (Gibco, USA) supplemented with 1× Glutamax (Gibco, USA), 10% FBS, 1× Penicillin–Streptomycin (Life technology, USA) and IL-2 (200 U/ml, PeproTech, USA); and stimulated with human CD3/CD28 Dynabeads (Life Technologies, USA) following the manufacturer’s instructions.

Article Title: PD-L1 and OX40 binding proteins for cancer regulation
Article Snippet: The anti-CD3 antibody (OKT3, BioLegend Cat. No. 317304) and anti-OX40 antibody leads (clones B6, B70, B120, A4, B17, B19, and B30), reference antibody (GSK3174998) or isotype antibodies (iso #1, #2) were coated in the Maxisorp 96-well plate. .. Meanwhile, naïve human CD3+ T-cells were isolated from the human blood from heathy adult volunteers using a commercially available RosetteSepTM Human T Cell Enrichment Cocktail (STEMCELL Cat. No. 15061) as manufacture's described. .. The isolated CD3+ T cells were then labeled by CFSE (CellTraceTM CFSE cell proliferation kit, Life technologies, Cat. No. C34554) and seeded as 1×106 cells/mL into the antibody pre-coated well containing RPMI 1640 medium, 10% fetal bovine serum and 2.5 mM L-glutamine to determine the cell proliferation and cytokine production.

Article Title: An Evaluation of the Potential Radiosensitization Effect of Spherical Gold Nanoparticles to Induce Cellular Damage Using Different Radiation Qualities
Article Snippet: .. In total, CD3 + T cells were isolated from peripheral blood using the RosetteSepTM Human T Cell Enrichment Cocktail (Stemcell Technologies) (Vancouver, BC, Canada) by negative selection after density gradient centrifugation (Density: 1.081 g/mL, RosetteSepTM Density Medium, Stemcell Technologies, Vancouver, BC, Canada). ..

Article Title: Chimeric PD‑1 receptor redirects primary T cells against childhood solid tumors but not to PD‑1 ligand‑positive CD80‑coexpressing cells.
Article Snippet: .. Peripheral blood mononuclear cells were collected from healthy volunteers after obtaining written informed consent, and T cells were isolated using rosetteSepTM Human T cell enrichment cocktail (STeMcell Technology, Vancouver, Bc, canada). .. T cells were incubated for 48‐72 h with dynabeads human T‐activator CD3/CD28 (Thermo Fisher Scientific, Waltham, Ma, uSa) in the presence of 200 iu/ml recombinant human interleukin‐2 (rhil‐2; Peprotech, cranbury, nJ, uSa) in rPMi‐1640 with 10% FBS. activated T cells were then transduced with retronectin (Takara, otsu, Japan) according to the manufacturer's instructions, with slight modifications.

Article Title: An Evaluation of the Potential Radiosensitization Effect of Spherical Gold Nanoparticles to Induce Cellular Damage Using Different Radiation Qualities
Article Snippet: .. In total, CD3+ T cells were isolated from peripheral blood using the RosetteSepTM Human T Cell Enrichment Cocktail (Stemcell Technologies) (Vancouver, BC, Canada) by negative selection after density gradient centrifugation (Density: 1.081 g/mL, RosetteSepTM Density Medium, Stemcell Technologies, Vancouver, BC, Canada). ..

Article Title: Chimeric PD-1 receptor redirects primary T cells against childhood solid tumors but not to PD-1 ligand-positive CD80-coexpressing cells
Article Snippet: .. Peripheral blood mononuclear cells were collected from healthy volunteers after obtaining written informed consent, and T cells were isolated using RosetteSepTM Human T Cell Enrichment Cocktail (STEMCELL Technology, Vancouver, BC, Canada). .. T cells were incubated for 48–72 h with Dynabeads human T-activator CD3/CD28 (Thermo Fisher Scientific, Waltham, MA, USA) in the presence of 200 IU/ml recombinant human interleukin-2 (rhIL-2; Peprotech, Cranbury, NJ, USA) in RPMI-1640 with 10% FBS.

Article Title: Self-replicating RNA and uses thereof
Article Snippet: .. Simultaneous isolation and enrichment was carried out using SepMateTM-50 columns containing LymphoprepTM and RosetteSepTM Human T Cell Enrichment Cocktail (STEMCELL TECHNOLOGIES). ..

other:

Article Title: PD-L1 mediated T cell inhibition by regulatory plasma cells induced after sepsis and COVID-19
Article Snippet: In humans, healthy volunteers’ T cells were isolated from whole blood by immunodensity isolation (RosetteSep Human T Cell Enrichment Cocktail; STEMCELL).

Selection:

Article Title: An Evaluation of the Potential Radiosensitization Effect of Spherical Gold Nanoparticles to Induce Cellular Damage Using Different Radiation Qualities
Article Snippet: .. In total, CD3 + T cells were isolated from peripheral blood using the RosetteSepTM Human T Cell Enrichment Cocktail (Stemcell Technologies) (Vancouver, BC, Canada) by negative selection after density gradient centrifugation (Density: 1.081 g/mL, RosetteSepTM Density Medium, Stemcell Technologies, Vancouver, BC, Canada). ..

Article Title: An Evaluation of the Potential Radiosensitization Effect of Spherical Gold Nanoparticles to Induce Cellular Damage Using Different Radiation Qualities
Article Snippet: .. In total, CD3+ T cells were isolated from peripheral blood using the RosetteSepTM Human T Cell Enrichment Cocktail (Stemcell Technologies) (Vancouver, BC, Canada) by negative selection after density gradient centrifugation (Density: 1.081 g/mL, RosetteSepTM Density Medium, Stemcell Technologies, Vancouver, BC, Canada). ..

Gradient Centrifugation:

Article Title: An Evaluation of the Potential Radiosensitization Effect of Spherical Gold Nanoparticles to Induce Cellular Damage Using Different Radiation Qualities
Article Snippet: .. In total, CD3 + T cells were isolated from peripheral blood using the RosetteSepTM Human T Cell Enrichment Cocktail (Stemcell Technologies) (Vancouver, BC, Canada) by negative selection after density gradient centrifugation (Density: 1.081 g/mL, RosetteSepTM Density Medium, Stemcell Technologies, Vancouver, BC, Canada). ..

Article Title: An Evaluation of the Potential Radiosensitization Effect of Spherical Gold Nanoparticles to Induce Cellular Damage Using Different Radiation Qualities
Article Snippet: .. In total, CD3+ T cells were isolated from peripheral blood using the RosetteSepTM Human T Cell Enrichment Cocktail (Stemcell Technologies) (Vancouver, BC, Canada) by negative selection after density gradient centrifugation (Density: 1.081 g/mL, RosetteSepTM Density Medium, Stemcell Technologies, Vancouver, BC, Canada). ..



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